Collaborative study with the Meier Lab @Imperial College London out in Science Advances

An exciting collaboration with the lab of Thomas Meier at Imperial College London has come to fruition, in the shape of a beautiful paper on the ATP synthase from the ‘dirty dozen’ bacterium Acinetobacter baumannii.

Check it out here, if you are interested in new ways of tackling antibiotic resistance:

We solved the structure of the fungal plasma membrane proton pump!

We did it! After over a decade of work, we finally determined the first ‘high’ (3.2Å) resolution structure of the fungal plasma membrane proton pump, Pma1.

A heroic effort by postdoc Sabine Heit and enriched by exciting molecular dynamics simulations by DPhil student Maxwell Geurts.

Check out the paper in Science Advances here:

N. crassa Pma1 hexamers embedded in a model membrane. Illustration made with Blender (thanks to Anthony Cheuk, Imperial College London).

Update, March 2022

Time is flying, and as I am about to come back from maternity leave, it is time for some important updates to be mentioned. New people have started in the lab, and publications have come out – the lab is buzzing with activity! I’ll dedicate individual posts to the big news shortly!

New Publication out in Cell Chemical Biology!

Excited to share the news of our newest publication:

Blockade of Oncogenic NOTCH1 with the SERCA Inhibitor CAD204520 in T Cell Acute Lymphoblastic Leukemia.

Marchesini M, Gherli A, Montanaro A, Patrizi L, Sorrentino C, Pagliaro L, Rompietti C, Kitara S, Heit S, Olesen CE, Møller JV, Savi M, Bocchi L, Vilella R, Rizzi F, Baglione M, Rastelli G, Loiacono C, La Starza R, Mecucci C, Stegmaier K, Aversa F, Stilli D, Lund Winther AM, Sportoletti P, Bublitz M, Dalby-Brown W, Roti G. Cell Chem Biol. 2020 Apr 15:S2451-9456(20)30116-1. doi: 10.1016/j.chembiol.2020.04.002. Online ahead of print. PMID: 32386594

Congratulations to Sabine Heit who contributed the SERCA-CAD204520 complex crystal structure. See also her related poster here in our gallery

A great collaboration with University of Parma and CaDo Biotech, Copenhagen.

Welcome new lab members!

This year has been way to busy – and the MBU-Lab team has grown! It’s about time to give a proper welcome to our new lab members!

Sam Bannon has started his DPhil this fall, and Max Geurts has joined us for a 6 month rotation at the start of his Wellcome Trust Doctoral Training Programme.

Matthew Hankins has decided to do his 18-week-long 4th year ‘Part II’ project in MBU-Lab, and Katja Schlegel has enriched us with her chemistry background, vising on a 4 month ERASMUS placement from the University of Münster, Germany.

It’s great to have you on the team!

News Highlight: Study on novel proton pump inhibitors

In a new research article published in PLOS ONE, work from the Bublitz lab and the Danish company PCovery has revealed a new class of small molecule compounds, so-called tetrahydrocarbazoles, that are potent inhibitors of the fungal proton pump. Picture1These compounds can cause a breakdown of the membrane potential in fungal cells and inhibit the growth of fungi in laboratory cultures.

Structural analysis suggests the compounds could block the ion entry pathway of the pump.

Check out our newest paper here: